Anatomy of a Motherboard Article by Marty Sems Lori Garris PDF

By Marty Sems Lori Garris

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In contrast to intracellular and patch-clamp electrophysiological techniques, this mode allows stable recordings of healthy neurons for long periods of time necessary to complete pharmacological experiments. Infrared differential interference videomicroscopy was used to locate VLPO neurons according to their typical size and triangular shape. 1). 1). 1 Pharmacological identification of the Type-1 and Type-2 neurons of the VLPO. (A1–B1) Microphotographies showing the typical morphology (triangular shaped) of the Type-1 and Type-2 neurons respectively, as observed in slices by means of the infrared differential interference videomicroscopy.

Fm Page 33 Tuesday, August 24, 2004 1:46 PM waking and PS and may accordingly stimulate theta in addition to gamma during these states. A small proportion of cells (<10%) recorded in the basal forebrain discharged at lower average rates with cortical activation than cortical slow wave activity and thus at lower rates during wake and/or PS than SWS in head-fixed rats32 in a manner similar to that of identified GABAergic Off cells in urethane-anesthetized rats (above). Commonly discharging maximally in SWS (called S-max), these cells nonetheless had varying properties.

As evident in the recording (see expanded trace of 500 msec period of unit activity in B [a]), the ISIH (D) and ACH (E), the unit discharges in a distinctly phasic manner with high frequency bursts (114 Hz peak frequency of the principal mode of the ISIH). This bursting occurs maximally during SWS, although it is also evident (by central peak in ACHs) during other states with a much lower incidence. 8 Hz) as in aW. 38). 5 for general details. , J. fm Page 35 Tuesday, August 24, 2004 1:46 PM two seemingly opposing cell groups are comprised of cholinergic and particular GABAergic neurons, respectively.

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Anatomy of a Motherboard Article by Marty Sems Lori Garris


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