By Yutaka Kawarabayasi Ph.D. (auth.), Pratyoosh Shukla, Brett I. Pletschke (eds.)
Enzyme expertise is one the main promising disciplines in glossy biotechnology. during this ebook, the purposes of a large choice of enzymes are highlighted. present stories in enzyme expertise are targeted in the direction of the invention of novel enzymes (termed “bio-discovery” or “bio-prospecting”) and the id and elucidation of novel pathways of those novel enzymes with emphasis on their commercial relevance. With the advance of molecular recommendations and different bioinformatics instruments, the time to combine this topic with different fields within the existence sciences has arrived. A swift growth of the data base within the box of enzyme biotechnology has happened over the last few years. a lot of this growth has been pushed via the bio-discovery of many new enzymes from a variety of environments, a few severe in nature, via next protein (enzyme) engineering. those enzymes have came upon a variety of purposes, starting from bioremediation, bio-monitoring, biosensor improvement, bioconversion to biofuels and different biotechnologically vital value-added items. Hydrolases represent an incredible element of the worldwide annual profit generated by way of commercial enzymes and the emphasis has accordingly been put on those enzymes and their functions. With the vast curiosity of researchers energetic during this sector, this booklet will serve to supply info on present features during this box of research. within the present variation, the contributions of many assorted issues in the direction of setting up new instructions of study within the quarter of enzyme biotechnology are defined. This ebook serves to supply a special resource of knowledge to undergraduates, put up graduates and doctoral classes in microbiology and biotechnology in addition to allied lifestyles sciences. the current version of the ebook covers all vital components of enzyme biotechnology i.e. the wide range of enzymes within the box of enzyme biotechnology and their business purposes, new tools and state of the art details on smooth tools of enzyme discovery. This ebook will act pretty much as good source on lots of the present elements of enzyme know-how for all scholars engaged in bioengineering and biotechnology.
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Extra info for Advances in Enzyme Biotechnology
Oryzae β-Galactosidase We have studied the synthesis of GOS catalyzed by a preparation of β-galactosidase from A. 1 M). We analyzed by HPAEC-PAD the product specificity of A. oryzae β-galactosidase (Urrutia et al. 2013). 10 shows the chromatogram obtained with this enzyme after 4 h of reaction. The main galactooligosaccharide synthesized by this enzyme was the trisaccharide 6-galactosyllactose (peak 8). Several disaccharides containing different bonds were also identified: Gal-β(1 → 6)-Gal (peak 3), Gal-β(1 → 6)-Glc (peak 4 ), Gal-β(1 → 3)-Gal (peak 7 ), and B.
Peaks 1, 2, and 5 were assigned to galactose, glucose, and lactose, respectively. We found that the most abundant GOS synthesized by this enzyme corresponded to peak 3 (the disaccharide 6-galactobiose [Gal-β(1 → 6)-Gal]), peak 4 (allolactose, Gal-β(1 → 6)-Glc), and peak 8. Purification of peak 8 was performed by semipreparative HPLC-HILIC. Its mass spectrum indicated that it was a trisaccharide, and its 2D-NMR spectra showed a galactosyl moiety β-(1 → 6) linked to the galactose ring of lactose (Rodriguez-Colinas et al.
2011a) described in Biolacta four isoforms differing in their molecular size: β-gal-A (189 kDa), β-gal-B (154 kDa), β-gal-C (134 kDa), and β-gal-D (91 kDa). The transferase activity of β-galactosidase from B. circulans has been also applied to the synthesis of lactosucrose (Wei et al. 2009), N-acetyl-lactosamine (Bridiau et al. 2010), and other galactosylated derivatives (Farkas et al. 2003). Interestingly, the enzyme is also able to catalyze the galactosylation of different acceptors in the presence of high percentages of organic cosolvents, up to 50 % v/v (Usui et al.
Advances in Enzyme Biotechnology by Yutaka Kawarabayasi Ph.D. (auth.), Pratyoosh Shukla, Brett I. Pletschke (eds.)